Immobilization of 4-MBA & Cu2+ on Au nanoparticles modified screen-printed electrode for glyphosate detection

Wei Li; Hongqi Wu; Cong Wu; Peidong Jiao; Ling Xu; Haiyan Song

doi:10.1016/j.talanta.2025.127530

Immobilization of 4-MBA & Cu²⁺ on Au nanoparticles modified screen-printed electrode for glyphosate detection

Talanta. 2025 Jan 10:286:127530. doi: 10.1016/j.talanta.2025.127530. Online ahead of print.

Authors

Wei Li¹, Hongqi Wu², Cong Wu³, Peidong Jiao², Ling Xu⁴, Haiyan Song⁵

Affiliations

¹ College of Agricultural Engineering, Shanxi Agricultural University, Taigu, 030801, China; Dryland Farm Machinery Key Technology and Equipment Key Laboratory of Shanxi Province, Taigu, 030801, China. Electronic address: allen@sxau.edu.cn.
² College of Agricultural Engineering, Shanxi Agricultural University, Taigu, 030801, China.
³ Department of Urology and Institutes for Systems Genetics, West China Hospital, Sichuan University, Chengdu, 610041, China.
⁴ Shanxi Hongrunqing Environmental Protection Technology Co., Ltd, Taiyuan, 030000, China.
⁵ College of Agricultural Engineering, Shanxi Agricultural University, Taigu, 030801, China; Dryland Farm Machinery Key Technology and Equipment Key Laboratory of Shanxi Province, Taigu, 030801, China.

PMID: 39798418
DOI: 10.1016/j.talanta.2025.127530

Abstract

This study introduces an innovative electrochemical biosensor, engineered through the functionalization screen-printed electrode (SPE) with a coordination complex comprised of 4-mercaptobenzoic acid (4-MBA) and copper ions (Cu²⁺), achieving precise quantitative determination of glyphosate. Electrodepositing gold nanoparticles (AuNPs) onto the electrode surface, forming a self-assembled monolayer (SAM) of 4-MBA via thiol-gold interactions, and immobilizing Cu²⁺ via coordination bonding with the monolayer, finalizing the electrochemical biosensor construction as Cu²⁺/4-MBA/AuNPs/SPE. The successful modification of the biosensor interface is confirmed through scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), and electrochemical characterization. Through parameter optimization, critical metrics for the biosensor preparation process have been determined. Using square wave voltammetry (SWV), a linear relationship between the glyphosate concentration and the peak current inhibition ratio at the electrode surface is established. Additionally, the repeatability and anti-interference capabilities of the fabricated biosensors are evaluated. The experimental outcomes affirm the biosensor's capability for quantitative glyphosate detection across a 5-100 nM range, boasting a 1.65 nM limit of detection (LOD). Testing on tap water samples verifies a robust recovery rate for glyphosate residues, spanning 89.84 %-107.48 %. The proposed biosensor holds significant promise for glyphosate detection, offering substantial applicability and this study provides a valuable reference for the advancement of biosensors geared toward the quantitative assessment of organophosphate pesticides (OPs).

Keywords: 4-Mercaptobenzoic acid (4-MBA); Electrochemical sensor; Glyphosate; Self-assembled monolayer (SAM); Square wave voltammetry (SWV).