Molecular cloning, expression, and functional analyses of plasmanylethanolamine desaturase gene of Takifugu rubripes

Hong-Long Ji; Chang Liu; Jing-Jing Zhang; Lei Lin; Qi Yang; Yu Yang; Cai-Chao Dong; Yang Bin He; Changwei Shao

doi:10.1016/j.gene.2025.149242

Molecular cloning, expression, and functional analyses of plasmanylethanolamine desaturase gene of Takifugu rubripes

Gene. 2025 Jan 10:149242. doi: 10.1016/j.gene.2025.149242. Online ahead of print.

Authors

Hong-Long Ji¹, Chang Liu², Jing-Jing Zhang³, Lei Lin⁴, Qi Yang⁵, Yu Yang⁶, Cai-Chao Dong⁷, Yang Bin He⁸, Changwei Shao⁹

Affiliations

¹ College of Fisheries, Zhejiang Ocean University, Zhoushan 316022, China. Electronic address: 18733601983@163.com.
² State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong 266071, China. Electronic address: liuchang707@126.com.
³ State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong 266071, China. Electronic address: jingjingZhang0307@163.com.
⁴ State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong 266071, China. Electronic address: linleicaas@163.com.
⁵ State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong 266071, China. Electronic address: yangqi_1999@163.com.
⁶ State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong 266071, China. Electronic address: yyu13156168974@163.com.
⁷ State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong 266071, China. Electronic address: bingo0635@163.com.
⁸ State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong 266071, China. Electronic address: heyb1002@163.com.
⁹ State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong 266071, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao 11 Marine Science and Technology Center, Qingdao, Shandong 266237, China. Electronic address: shaocw@ysfri.ac.cn.

PMID: 39800193
DOI: 10.1016/j.gene.2025.149242

Abstract

The aging population has led to a significant increase in neurodegenerative diseases, particularly Alzheimer's disease (AD), which adversely affects the quality of life and longevity of the elderly. Abnormal plasmalogen metabolism plays a crucial role in the pathogenesis of AD. This study focused ontmem189, a key gene involved in plasmalogen synthesis. We successfully cloned and characterized the open reading frame (ORF) oftmem189, revealing that it encodes a protein consisting of 275 amino acids. Notably,tmem189expression was found to be highest in liver tissues compared to other tissues. We transfected a GFP-fused eukaryotic expression vector into 293 T cells, confirming successful expression oftmem189with increased relative levels. Additionally, liquid chromatography-mass spectrometry (LC-MS) analysis demonstrated thattmem189promotes plasmalogen synthesis in the transfected 293 T cells. Our findings suggest thattmem189could serve as a potential target for the treatment of neurodegenerative diseases, providing new insights into the promotion of plasmalogen synthesis.

Keywords: Cloning; Expression; Plasmalogen; Tmem189.