Biological control, which utilizes nematophagous fungi to reduce gastrointestinal nematode populations, may effectively diminish the need for chemical anthelmintic treatments. However, the limited knowledge surrounding the mass production of chlamydospores hinders the widespread use of biological products as alternatives to traditional anthelmintics. This study aimed to evaluate the development of liquid culture media for the large-scale production of the nematophagous fungi Duddingtonia flagrans using a systematic procedure, progressing from microplates to bioreactor. The liquid culture media were successfully validated in a 13 L bioreactor, achieving a yield of 2.18x107 chlam/g per day, which is comparable to the standard process of solid-state fermentation (SSF). Moreover, the nematode predatory ability remained unaffected by the changes in scales and exhibited a superior efficacy of over 90%. Consequently, this study demonstrates that the submerged fermentation approach serves as a viable alternative for the mass production of nematophagous fungi like D. flagrans.
Keywords: Biolog-Microplate FF test; Submerged fermentation; bioreactor; chlamydospores; nematodes.
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