TRIB1 facilitates the proliferation and migration of ovarian cancer cells by inducing EMT progression

Guangyan Shi; Kristian Holgersson; Zhen Xin; Laszlo Szekely; Qiqiao Du; Xu Jing

doi:10.14670/HH-18-868

TRIB1 facilitates the proliferation and migration of ovarian cancer cells by inducing EMT progression

Histol Histopathol. 2024 Dec 31:18868. doi: 10.14670/HH-18-868. Online ahead of print.

Authors

Guangyan Shi¹, Kristian Holgersson², Zhen Xin¹, Laszlo Szekely³, Qiqiao Du⁴, Xu Jing⁵

Affiliations

¹ Medical Laboratory center, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, PR China.
² Loma Linda University, CA, USA.
³ Department of Pathology/Cytology, Karolinska University Laboratory, Stockholm, Sweden.
⁴ Department of Obstetrics and Gynecology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, PR China.
⁵ Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Solna, Sweden. Wanghl2019188@126.com.

PMID: 39807763
DOI: 10.14670/HH-18-868

Abstract

Aim: Ovarian cancer (OC) is a fatal female malignant tumor that severely impacts the health of women worldwide. Due to the lack of diagnostic biomarkers, 70% of OC patients are considered in the advanced stage at the first diagnosis. Exploring novel biomarkers for OC diagnosis has become an urgent clinical need to address. TRIB1 is a newly discovered oncogene in several malignant tumors, including acute myeloid leukemia, prostate cancer, and breast cancer. However, the biological function of TRIB1 in OC remains uncertain and, therefore, was explored in the present study.

Methods: Levels of TRIB1 in OC and normal tissues were evaluated in the GEPIA database. TRIB1-KD was constructed in ES-2 cells and TRIB1-OE was constructed in OVCAR3 cells using a siRNA and OE vector, respectively. The proliferation ability was determined using the CCK-8 and clone formation assays. The migration ability was detected using the wound healing and Transwell assays. The expression of epithelial-mesenchymal transition (EMT) biomarkers was determined using western blotting.

Results: TRIB1 was markedly upregulated in OC tissues compared with normal ovarian tissues in the GEPIA database. The TRIB1 level was slightly altered among ES-2, CAOV3, and SKOV3 cells, with the highest expression in ES-2 cells, which was greatly reduced in OVCAR3 cells. In TRIB1-KD ES-2 cells, a remarkably reduced proliferation ability was observed with the CCK-8 and clone formation assays, accompanied by a reduction in migration distance in the Wound healing assay and the number of migrated cells in the Transwell assay. In contrast, in TRIB1-OE OVCAR3 cells, increased proliferation ability was observed, accompanied by increased migration distance and number of migrated cells. Furthermore, EMT progression was markedly repressed in TRIB1-KD ES-2 cells and remarkably enhanced in TRIB1-OE OVCAR3 cells.

Conclusion: TRIB1 facilitated the proliferation and migration of OC cells by enhancing EMT progression.