Atp24δ8, a p24 family member, regulates the unfolded protein response and ER stress tolerance in Arabidopsis

Fayin He; Yue Zeng; Yin An; Yangnian Pan; Jiana Zhu; Chunpeng Fei; Guishuang Tian; Yin Yi; Ming Tang; Kun Li; Zhengting Yang

doi:10.1016/j.ijbiomac.2025.139776

Atp24δ8, a p24 family member, regulates the unfolded protein response and ER stress tolerance in Arabidopsis

Int J Biol Macromol. 2025 Jan 12:139776. doi: 10.1016/j.ijbiomac.2025.139776. Online ahead of print.

Authors

Fayin He¹, Yue Zeng¹, Yin An¹, Yangnian Pan¹, Jiana Zhu¹, Chunpeng Fei¹, Guishuang Tian¹, Yin Yi¹, Ming Tang¹, Kun Li², Zhengting Yang³

Affiliations

¹ Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Karst Mountainous Areas of Southwestern China, School of Life Sciences, Guizhou Normal University, Guiyang 550025, China.
² Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Karst Mountainous Areas of Southwestern China, School of Life Sciences, Guizhou Normal University, Guiyang 550025, China. Electronic address: KunLi@gznu.edu.cn.
³ Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Karst Mountainous Areas of Southwestern China, School of Life Sciences, Guizhou Normal University, Guiyang 550025, China. Electronic address: zhengtingyang@gznu.edu.cn.

PMID: 39809389
DOI: 10.1016/j.ijbiomac.2025.139776

Abstract

ER stress activates the unfolded protein response (UPR), a critical mechanism for maintaining cellular homeostasis in plants. The p24 protein family is known to be involved in protein trafficking between the endoplasmic reticulum (ER) and the Golgi apparatus, but its role in ER stress remains unclear in plants. In this study, we found that Atp24δ8(delta8), a member of the δ-2 subclass of the p24 family, is significantly upregulated in response to tunicamycin-induced ER stress. Subcellular localization confirmed that Atp24δ8 resides in the ER, and CRISPR-Cas9 loss-of-function mutants displayed increased sensitivity to ER stress. Further analysis showed that Atp24δ8 regulates key UPR genes, highlighting its role in promoting ER stress tolerance. These findings provide new insights into the molecular mechanisms that plants use to cope with ER stress, potentially contributing to the development of stress-tolerant crops.

Keywords: Endoplasmic reticulum stress; Unfolded protein response(p24); p24 family.