Transcription factors PHR1 and PHR1-like 1 regulate ABA-mediated inhibition of seed germination and stomatal opening in Arabidopsis

Plant Sci. 2025 Jan 16:352:112389. doi: 10.1016/j.plantsci.2025.112389. Online ahead of print.

Abstract

Low phosphate (LP) availability significantly impacts crop yield and quality. PHOSPHATE STARVATION RESPONSE1 (PHR1) along with PHR1-like 1 (PHL1) act as a key transcriptional regulator in a plant's adaptive response to LP conditions. Abscisic acid (ABA) plays an important role in how plants respond to environmental stresses like salinity and drought. However, the involvement of PHR1 and PHL1 in ABA response and signalling mechanisms remains to be fully understood. Our findings reveal that PHR1 and PHR1/PHL1 knockout mutations enhance the responsiveness of seed germination, early seedling growth, and stomatal opening to ABA in Arabidopsis. Furthermore, these mutations increase sensitivity to combined LP and ABA stress. In contrast, overexpression of PHR1 or PHL1 reduces this sensitivity in Arabidopsis. Knockout mutations of PHR1 and PHR1/PHL1 also increase sensitivity to salt and osmotic stresses, as well as to combined LP and salinity/osmotic stress, while overexpression of PHR1 or PHL1 reduces their sensitivity in seed germination and early seedling development. Knockout mutations of SPX1 and SPX2, negative regulators of PHR1 and PHL1, decrease sensitivity to ABA and salt/osmotic stresses in Arabidopsis. A group of genes related to ABA metabolism and signalling is significantly affected by the knockout or overexpression of PHR1 and PHL1, with a large proportion of these genes containing PHR1 binding site (P1BS) in their promoters. Moreover, the ABA-sensitive phenotype of phr1 or phr1 phl1 mutants can be rescued by PHR1 homologs from chlorophyte algae, liverwort and rice, suggesting their conserved roles in ABA signalling. These results indicate that PHR1 and its homologs negatively regulate plant responses to ABA in seed germination and stomatal aperture. This study provides new insights into the interplay between Pi homeostasis, abiotic stress and ABA signaling. Moderately increasing the expression of PHR1 or its homologs in crops could be a potential strategy to enhance plant resistance to combined LP and osmotic stress.

Keywords: ABA response; ABA signaling; Combined stresses; Low phosphorus; Osmotic stress; Phosphate starvation response; Salt stress.