The NADP binding site on rabbit muscle aldolase

Biochem Int. 1985 Jan;10(1):53-62.

Abstract

Rabbit muscle aldolase binds NADPH with a 1:1 stoichiometry and with a dissociation constant 18 microM. Three sites of the dinucleotide are involved in the binding: the adenosyl diphosphate moiety, the nicotinamide-ribose, and the nicotinamide ring. These data show the existence of a specific dinucleotide binding site in the aldolase molecule.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Binding Sites
  • Chemical Phenomena
  • Chemistry
  • Fructose-Bisphosphate Aldolase / antagonists & inhibitors
  • Fructose-Bisphosphate Aldolase / metabolism*
  • In Vitro Techniques
  • Muscles / metabolism*
  • NADP / metabolism*
  • Rabbits

Substances

  • NADP
  • Fructose-Bisphosphate Aldolase