Methotrexate binding to dihydrofolate reductase from a methotrexate-resistant strain of Escherichia coli

J Biol Chem. 1973 Sep 25;248(18):6375-9.
No abstract available

MeSH terms

  • Binding Sites
  • Chromatography, Affinity
  • Chromatography, Gel
  • Chromatography, Ion Exchange
  • Drug Resistance, Microbial
  • Escherichia coli / drug effects
  • Escherichia coli / enzymology*
  • Folic Acid
  • Folic Acid Antagonists
  • Kinetics
  • Methotrexate / metabolism*
  • Methotrexate / pharmacology
  • NADP
  • Osmolar Concentration
  • Oxidation-Reduction
  • Protein Binding
  • Spectrometry, Fluorescence
  • Spectrophotometry, Ultraviolet
  • Tetrahydrofolate Dehydrogenase / metabolism*
  • Time Factors

Substances

  • Folic Acid Antagonists
  • NADP
  • Folic Acid
  • Tetrahydrofolate Dehydrogenase
  • Methotrexate