The subunits of chemically treated proteoglycan isolated from bovine nasal cartilage

Biochem J. 1971 Mar;122(1):101-6. doi: 10.1042/bj1220101.

Abstract

1. The light fraction of the proteoglycan of bovine nasal cartilage was split by treatment with 0.1m-hydrochloric acid in acetone. The products were separated by gel filtration on 4% agarose and two retarded fractions were detected and isolated. These two fractions were found to have a Stokes radius of 134 and 47 A respectively, as determined by calibration of the column against proteins of known hydrodynamic volumes. 2. The 47 A fraction had a protein content of 4% and a glucosamine/galactosamine ratio 1:23. The 134 A fraction had a protein content of 20% and a glucosamine/galactosamine ratio 1:4.8. 3. The results of the viscometric studies on both fractions suggested that the 134 A fraction alone exhibited the property of undergoing reversible pH-dependent aggregation with a transition point at pH4.9. 4. It was concluded that these fractions could represent subunits of the native cartilage proteoglycan.

MeSH terms

  • Acetone
  • Animals
  • Cartilage / analysis*
  • Cattle
  • Chromatography, Gel
  • Galactosamine / analysis
  • Glucosamine / analysis
  • Glycoproteins / analysis*
  • Glycosaminoglycans / analysis*
  • Hydrochloric Acid
  • Hydrogen-Ion Concentration
  • Microscopy, Electron
  • Nasal Septum
  • Osmotic Pressure
  • Viscosity

Substances

  • Glycoproteins
  • Glycosaminoglycans
  • Acetone
  • Galactosamine
  • Glucosamine
  • Hydrochloric Acid