Unusual cytoplasmic inclusion bodies in medullary carcinoma of the thyroid gland

Acta Pathol Jpn. 1982 Mar;32(2):319-26. doi: 10.1111/j.1440-1827.1982.tb02052.x.

Abstract

Medullary carcinoma of the thyroid from a 43-year-old patient showed 4 types of unusual cytoplasmic inclusion bodies ultrastructurally. Type 1 inclusion is composed of rod and doughnut-shaped bodies aggregated in the cytoplasma and not enclosed by a limiting membrane. Typical neurosecretory granules are intermingled with the rod and doughnut-shaped bodies. The doughnut-shaped bodies often have an electron dense core which is similar to the neurosecretory granules. The rod-shaped bodies are bounded by trilaminar unit membrane and have a slightly electron dense matrix. A centriole is often found associated with this type of inclusion. Type 2 inclusion is composed of small secretory granules and clear vesicles. These inclusions can be found either with or without surrounding membrane. Type 3 inclusion is a large osmiophilic body, or secondary lysosome, in which some degenerated organelles are discernible. Type 4 inclusion is a fragmented nucleus with condensation and margination of the chromatin, so-called karyorrhexis. Routine hematoxylin and eosin sections did not show each type of inclusion but semithin sections stained with Paragon suggested 4 different types of inclusions. Type 3 and 4 inclusions represent forms of degeneration and cell death in the tumor cells and probably represent a remote effect of radiation. Type 1 inclusion is not commonly associated with cell death and may represent a unique organellar aberration in these tumor cells. Type 2 inclusion is simply an aggregation of secretory granules and clear vesicles.

Publication types

  • Case Reports

MeSH terms

  • Adult
  • Carcinoma / ultrastructure*
  • Cell Nucleus / ultrastructure
  • Cytoplasm / ultrastructure*
  • Cytoplasmic Granules / ultrastructure
  • Humans
  • Inclusion Bodies / ultrastructure*
  • Lysosomes / ultrastructure
  • Male
  • Microscopy, Electron
  • Staining and Labeling
  • Thyroid Neoplasms / ultrastructure*