Proteins specified by avian erythroblastosis virus: coding region localization and identification of a previously undetected erb-B polypeptide

Proc Natl Acad Sci U S A. 1982 Jul;79(13):3958-62. doi: 10.1073/pnas.79.13.3958.

Abstract

Avian erythroblastosis virus (AEV) induces erythroblastosis and sarcomas in chickens. Two domains within the viral genome, erb-A and erb-B, have been implicated in AEV-mediated oncogenesis. By use of hybridization-arrested translations and hybridization-selections of mRNA, we have mapped on the viral genome the polypeptides specified by the erb domains. The results of hybridization-arrest with DNA representing the spliced 5' leader region of the AEV mRNA suggested that the authentic product of the erb-B domain was a 61,000 molecular weight protein, not a 41,000 molecular weight polypeptide previously identified.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alpharetrovirus / genetics*
  • Animals
  • Avian Leukosis Virus / genetics*
  • Cell Transformation, Viral
  • Chickens
  • Erythrocytes
  • Nucleic Acid Hybridization
  • Peptides / genetics*
  • Plasmids
  • Protein Biosynthesis*
  • RNA, Messenger / genetics
  • RNA, Viral / genetics

Substances

  • Peptides
  • RNA, Messenger
  • RNA, Viral