Intracellular transport of the transmembrane glycoprotein G of vesicular stomatitis virus through the Golgi apparatus as visualized by electron microscope radioautography

J Cell Biol. 1982 Jul;94(1):36-41. doi: 10.1083/jcb.94.1.36.

Abstract

The intracellular migration of G protein in vesicular stomatitis virus-infected cells was visualized by light and electron microscope radioautography after a 2-min pulse with [3H]mannose followed by nonradioactive chase for various intervals. The radioactivity initially (at 5-10 min) appeared predominantly in the endoplasmic reticulum, and the [3H]mannose-labeled G protein produced was sensitive to endoglycosidase H. Silver grains were subsequently (at 30-40 min) observed over the Golgi apparatus, and the [3H]mannose-labeled G protein became resistant to endoglycosidase H digestion. Our data directly demonstrate the intracellular transport of a plasmalemma-destined transmembrane glycoprotein through the Golgi apparatus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Autoradiography
  • Cell Membrane / metabolism
  • Cells, Cultured
  • Cricetinae
  • Endoplasmic Reticulum / metabolism
  • Glycoside Hydrolases / pharmacology
  • Golgi Apparatus / metabolism*
  • Mannose / metabolism
  • Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase
  • Mesocricetus
  • Mice
  • Microscopy, Electron
  • Vesicular stomatitis Indiana virus / physiology*
  • Viral Envelope Proteins
  • Viral Proteins / metabolism*

Substances

  • Viral Envelope Proteins
  • Viral Proteins
  • Glycoside Hydrolases
  • Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase
  • Mannose