Manipulation of anti-LDH-B response by T suppressor factors

J Immunol. 1984 Apr;132(4):1605-7.

Abstract

Hybridomas produced by fusion between the BW5147 thymoma and an LDH-B-specific B10.A(2R) suppressor T cell line secrete two T suppressor factors (TsF). One factor (TsF-A) shares Mhc determinants with the A alpha A beta molecule and suppresses proliferating Th cells; the other (TsF-E) shares determinants with the E alpha E beta molecule and it inhibits the maturation of the T suppressor (Ts) cells. Here we demonstrate that the two factors can be used to alter the immune response status of cultured T lymphocytes or of an animal. When added to a culture of LDH-B-primed cells or injected into mice, the TsF-A turns responders into nonresponders, presumably by blocking the proliferation of the Th cells. The TsF-E converts nonresponder cultures or mice into responders, presumably by preventing the differentiation of Ts cells. As there are good prospects for obtaining TsF in large quantities and in a highly purified form, this manipulation of the immune response by the deployment of specific factors promises to become an efficient new method of immunotherapy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens / administration & dosage
  • Antigens / immunology*
  • Dose-Response Relationship, Immunologic
  • Female
  • L-Lactate Dehydrogenase / immunology*
  • Lymphocyte Activation
  • Lymphokines / physiology*
  • Male
  • Mice
  • Mice, Inbred A
  • Suppressor Factors, Immunologic
  • T-Lymphocytes / immunology

Substances

  • Antigens
  • Lymphokines
  • Suppressor Factors, Immunologic
  • L-Lactate Dehydrogenase