Abstract
Most mutants selected for derepression of the biotin operon required elevated concentrations of biotin for growth. Mutant extracts were deficient in holoenzyme synthetase activity.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Apoproteins / metabolism
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Biotin / biosynthesis*
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Biotin / metabolism
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Biotin / pharmacology
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Carbon-Nitrogen Ligases*
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Escherichia coli / genetics*
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Escherichia coli / growth & development
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Escherichia coli / metabolism
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Genes, Regulator*
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Ligases / metabolism
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Mutation
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Repressor Proteins / biosynthesis
Substances
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Apoproteins
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Repressor Proteins
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Biotin
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Ligases
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Carbon-Nitrogen Ligases
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holocarboxylase synthetases