Rod transduction parameters from the a wave of local receptor populations

J Opt Soc Am A Opt Image Sci Vis. 1995 Oct;12(10):2259-66. doi: 10.1364/josaa.12.002259.

Abstract

The analysis of electroretinogram a waves from locally stimulated populations of rods is complicated by the presence of scattered light within the eye. Scattered-light and cone contributions can be assessed after brief flashes of light designed to saturate only rods in the locally stimulated area. Subtracting the scattered-light and the cone responses from the local electroretinogram gives a pure rod a wave that can be fitted with models of photoreceptor activity. We demonstrate the feasibility of this technique by recording local rod a waves from a group of five normal subjects and by fitting the a waves with the rod model to derive transduction parameters. The local rod a waves are compared with expected responses derived from simulations in which the response of the entire retina to heterogeneous illumination is mimicked.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Electroretinography
  • Humans
  • Light
  • Photic Stimulation
  • Photoreceptor Cells / physiology*
  • Retinal Cone Photoreceptor Cells / physiology
  • Retinal Rod Photoreceptor Cells / physiology*
  • Scattering, Radiation
  • Signal Transduction / physiology*