Sustained c-kit expression in a human erythroleukemia cell line (HEL) after megakaryocytic differentiation induced by 12-O-tetradecanoylphorbol-13-acetate (TPA)

Leuk Res. 1993 Dec;17(12):1079-82. doi: 10.1016/0145-2126(93)90167-j.

Abstract

Changes in c-kit proto-oncogene expression were examined in a human erythroleukemia cell line, HEL, during 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced megakaryocytic differentiation. When HEL cells were treated with 10(-7) M TPA, glycophorin A expression and hemoglobin synthesis were reduced, while the expression of GP IIb/IIIa was induced in association with the morphological changes. Northern blot analysis showed that, during this megakaryocytic differentiation of HEL cells, c-kit mRNA expression persisted even after there was an apparent reduction in c-myc mRNA. This finding supports the idea that the expression of c-kit, a marker of primitive hematopoietic progenitors, may persist along with differentiation toward a megakaryocytic lineage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cell Differentiation / drug effects*
  • Cell Division / drug effects
  • Cell Line
  • DNA Primers
  • Gene Expression Regulation, Neoplastic / drug effects*
  • Glycophorins / biosynthesis
  • Hemoglobins / biosynthesis
  • Humans
  • Kinetics
  • Leukemia, Erythroblastic, Acute
  • Megakaryocytes / cytology
  • Megakaryocytes / drug effects*
  • Megakaryocytes / metabolism
  • Molecular Sequence Data
  • Oligonucleotides, Antisense
  • Platelet Membrane Glycoproteins / biosynthesis
  • Polymerase Chain Reaction
  • Proto-Oncogene Mas
  • Proto-Oncogene Proteins / analysis
  • Proto-Oncogene Proteins / biosynthesis*
  • Proto-Oncogene Proteins c-kit
  • Proto-Oncogenes* / drug effects
  • Receptor Protein-Tyrosine Kinases / analysis
  • Receptor Protein-Tyrosine Kinases / biosynthesis*
  • Receptors, Colony-Stimulating Factor / analysis
  • Receptors, Colony-Stimulating Factor / biosynthesis*
  • Tetradecanoylphorbol Acetate / pharmacology*
  • Tumor Cells, Cultured

Substances

  • DNA Primers
  • Glycophorins
  • Hemoglobins
  • MAS1 protein, human
  • Oligonucleotides, Antisense
  • Platelet Membrane Glycoproteins
  • Proto-Oncogene Mas
  • Proto-Oncogene Proteins
  • Receptors, Colony-Stimulating Factor
  • Proto-Oncogene Proteins c-kit
  • Receptor Protein-Tyrosine Kinases
  • Tetradecanoylphorbol Acetate