Quantification of urinary catecholamines, their abundant metabolites, and 5-hydroxyindoleacetic acid by high performance liquid chromatography and electrochemical detection, using a single mobile phase and uniform isocratic conditions

Abstract

A simple and flexible isocratic HPLC procedure was developed for the measurement of catecholamines and their abundant metabolites ( e.g. nor- metanephrine, metanephrine, methoxy-tyramine, vanillylmandelic acid, homovanillic acid) and 5-hydroxyindolacetic acid by ion-pair reversed phase chromatography on C18 columns, using a single mobile phase containing both sodium octane sulphonate and diethylamine as ion-pairing reagents.