Abstract
We have investigated the capacity of interleukin (IL)-4 or stem cell factor (SCF) to induce direct mediator release from rodent peritoneal mast cells, and also to induce or regulate cytokine gene expression in the human HMC-1 mast cell line. SCF, but not IL-4, induced low levels of serotonin release from mouse or rat peritoneal mast cells; rat mast cells acquired enhanced responsiveness to SCF during culture. IL-4, but not SCF, enhanced ionomycin-induced transcription and secretion of several genes, including the cytokines IL-3, IL-4, granulocyte/macrophage-colony-stimulating factor, IL-8 and the receptor for IL-6 in the human HMC-1 mast cell line.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Line
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Gene Expression Regulation / drug effects*
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Granulocyte-Macrophage Colony-Stimulating Factor / biosynthesis*
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Granulocyte-Macrophage Colony-Stimulating Factor / genetics
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Hematopoietic Cell Growth Factors / pharmacology*
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Histamine Release / drug effects*
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Humans
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Interleukin-4 / pharmacology*
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Interleukins / biosynthesis*
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Interleukins / genetics
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Ionomycin / pharmacology
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Mast Cells / drug effects*
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Mast Cells / metabolism
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Mice
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Peritoneal Cavity / cytology
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Rats
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Receptors, Interleukin / biosynthesis*
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Receptors, Interleukin / genetics
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Receptors, Interleukin-6
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Serotonin / metabolism
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Stem Cell Factor
Substances
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Hematopoietic Cell Growth Factors
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Interleukins
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Receptors, Interleukin
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Receptors, Interleukin-6
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Stem Cell Factor
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Interleukin-4
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Serotonin
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Ionomycin
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Granulocyte-Macrophage Colony-Stimulating Factor