Abstract
To detect HIV-1 plasma viral load in seropositive patients, we applied a new molecular quantitation technique: branched DNA signal amplification (bDNA). We performed bDNA with 99 sera from HIV-1 seropositive patients undergoing antiretroviral therapy. We compared the results obtained with p24 antigenemia and CD4+ cell count. The bDNA proved quite sensitive and available for routine use in laboratories.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies, Monoclonal / immunology
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Blood / virology*
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CD4 Lymphocyte Count
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DNA Probes / genetics
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Enzyme-Linked Immunosorbent Assay
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Female
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HIV Core Protein p24 / analysis
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HIV Seropositivity / blood*
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HIV-1 / immunology
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HIV-1 / isolation & purification*
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Humans
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Luminescent Measurements
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Male
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Nucleic Acid Hybridization*
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RNA, Viral / isolation & purification*
Substances
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Antibodies, Monoclonal
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DNA Probes
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HIV Core Protein p24
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RNA, Viral