Possible errors in assay for beta-glycosidase activity

Appl Environ Microbiol. 1995 Feb;61(2):820-2. doi: 10.1128/aem.61.2.820-822.1995.

Abstract

Cecal homogenates were assayed for the enzymes beta-glucosidase, beta-glucuronidase, and beta-galactosidase. Anaerobic incubation with the addition of excess 3,4-dichloronitrobenzene, a substrate for nitroreductase, significantly increased the detection of the beta-glycosidase enzymes' activities.

MeSH terms

  • Aerobiosis
  • Anaerobiosis
  • Animals
  • Cecum / enzymology
  • Glucuronidase / analysis*
  • Male
  • Nitrobenzenes
  • Nitrophenols
  • Rats
  • Rats, Inbred F344
  • beta-Galactosidase / analysis*
  • beta-Glucosidase / analysis*

Substances

  • Nitrobenzenes
  • Nitrophenols
  • beta-Glucosidase
  • beta-Galactosidase
  • Glucuronidase
  • 4-nitrophenol