Genomic cloning of the human histo-blood group ABO locus

Biochem Biophys Res Commun. 1995 Jan 5;206(1):318-25. doi: 10.1006/bbrc.1995.1044.

Abstract

A P1 phage clone containing the near complete protein coding region of the human histo-blood group ABO gene locus was isolated and characterized. The insert was estimated at approximately 41 kp, and an approximately 20 kbp BamHI fragment contained the near complete coding region as evaluated by Southern blot analysis. Intron/exon boundaries were determined and the coding region was found to span at least seven exons. In situ hybridization to metaphase chromosomes confirmed the predicted localization to 9q34.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ABO Blood-Group System / biosynthesis
  • ABO Blood-Group System / genetics*
  • Animals
  • Base Sequence
  • Chromosome Mapping
  • Chromosomes, Human, Pair 9*
  • Cloning, Molecular
  • DNA Primers
  • Exons
  • Female
  • Hominidae / genetics*
  • Humans
  • In Situ Hybridization, Fluorescence
  • Male
  • Molecular Sequence Data
  • Oligonucleotide Probes
  • Organ Specificity
  • Polymerase Chain Reaction
  • Restriction Mapping
  • Testis / metabolism
  • Transcription, Genetic*

Substances

  • ABO Blood-Group System
  • DNA Primers
  • Oligonucleotide Probes

Associated data

  • GENBANK/X84746
  • GENBANK/X84747
  • GENBANK/X84748
  • GENBANK/X84749
  • GENBANK/X84750
  • GENBANK/X84751
  • GENBANK/X84752