Unique sequence STSs for 21 cytogenetically mapped loci on human chromosome 19

H W Mohrenweiser; S Tsujimoto; K Tynan; J Lamerdin; A V Carrano

doi:10.1159/000134063

Unique sequence STSs for 21 cytogenetically mapped loci on human chromosome 19

Cytogenet Cell Genet. 1995;71(1):58-61. doi: 10.1159/000134063.

Authors

H W Mohrenweiser¹, S Tsujimoto, K Tynan, J Lamerdin, A V Carrano

Affiliation

¹ Human Genome Center, Biology and Biotechnology Research Program L-452, Lawrence Livermore National Laboratory, Livermore, CA 94550, USA.

PMID: 7606929
DOI: 10.1159/000134063

Abstract

Sequence-tagged sites (STSs) are key elements in efforts to construct and integrate the various physical maps of a genome. We report the development of 21 STSs assigned to cosmids mapped to specific locations on human chromosome 19 by fluorescence in situ hybridization (FISH). At least one STS has been assigned to each band. The PCR primers generate a single product from genomic DNA. Each product is a single copy probe for Southern blots of human genomic DNA. YACs have been identified with 18 of these STSs.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Base Sequence
Chromosomes, Human, Pair 19*
DNA Primers
Humans
In Situ Hybridization, Fluorescence
Molecular Sequence Data
Sequence Tagged Sites*

Substances

DNA Primers