The locus coeruleus (LC) was anatomically separated from the periventricular gray substance (PVG) by means of knife cuts in the adult female rat presenting regular estrous cycling. This resulted in a transient suppression of the estrous cycling that lasted 10-13 days after surgery. After this period, irregular or regular cycling activity was observed. The regular cycling was restored 30-45 days after the knife cuts. Golgi impregnation of some of the brains of these rats revealed regenerative elements in the knife-cut-insulted area. Thus, blood vessels, macrophagic-like elements, and glial-like elements were observed in close relation with the knife-cut pathway. Additionally, well-defined stained neurons typical of the LC and PVG were observed in close proximity to the knife-cut pathway. Dendritic and axon projections towards the insulted area were observed. Well defined axons were seen across the knife-cut pathway. These data support, first, that the LC-PVG communication is part of a circuitry for the modulation of gonadotropic activity, and second, that in the restoration of the estrous cyclicity after the knife cut, regenerative processes leading to a LC-PVG functional reconnection occurred after the knife cut.