Chemokine binding and activities mediated by the mouse IL-8 receptor

J Immunol. 1995 Aug 15;155(4):2158-64.

Abstract

In humans and rabbits two similar IL-8R mediate the chemotaxis and activation of neutrophils induced by alpha chemokines. We present data to suggest that there is only one such IL-8R gene in mice. We then use mice with a targeted deletion of this gene to characterize alpha chemokine ligands that signal via the mouse IL-8R. These experiments show that mouse macrophage inflammatory protein 2 binds the receptor with high affinity (Kd = approximately 1.5 nM) and potently activates both an intracellular Ca2+ flux and a chemotactic response, events absent in neutrophils from receptor-deleted mice. Mouse KC is approximately 10-fold less potent. These results show that macrophage inflammatory protein 2 and KC potently activate mouse neutrophils via a unique IL-8R, and these proteins may function as the major proinflammatory alpha chemokines in mice.

MeSH terms

  • Animals
  • Calcium / metabolism
  • Chemokine CXCL1
  • Chemokine CXCL2
  • Chemokines
  • Chemokines, CXC
  • Cytokines / metabolism
  • Cytokines / pharmacology*
  • Humans
  • Interleukin-8 / metabolism
  • Interleukin-8 / pharmacology*
  • Mice
  • Mice, Inbred C57BL
  • Monokines / metabolism
  • Monokines / pharmacology*
  • Neutrophils / metabolism
  • Receptors, Interleukin / genetics
  • Receptors, Interleukin / physiology*
  • Receptors, Interleukin-8A

Substances

  • Chemokine CXCL1
  • Chemokine CXCL2
  • Chemokines
  • Chemokines, CXC
  • Cxcl1 protein, mouse
  • Cytokines
  • Interleukin-8
  • Monokines
  • Receptors, Interleukin
  • Receptors, Interleukin-8A
  • keratinocyte-derived chemokines
  • Calcium

Associated data

  • GENBANK/U31207