We have developed an algorithm, called multi-motor unit action potential analysis (MMA), to aid quantification in routine needle EMG examination. In only 5-8 min, it was possible to extract, analyze, and validate 20 motor unit action potentials (MUAPs). In the biceps muscle of normal subjects, the MUAP measurements are compared with measurements using manual, triggered averaging, automatic decomposition, and other MMA algorithms described in the literature. The mean values of MUAP features by MMA fell between the extreme limits obtained from the amplitude-triggered method and the manual method described in the literature. In patients, the results are consistent with routine EMG and similar to those results described in the literature. Fast acquisition and analysis of MUAPs as well as interference pattern (IP) analysis make this technique useful to document EMG abnormalities in routine needle examination.