Identification of two distinct chromosome 12-derived amplification units in neuroblastoma cell line NGP

N Van Roy; A Forus; O Myklebost; N C Cheng; R Versteeg; F Speleman

doi:10.1016/0165-4608(95)00034-m

Identification of two distinct chromosome 12-derived amplification units in neuroblastoma cell line NGP

Cancer Genet Cytogenet. 1995 Jul 15;82(2):151-4. doi: 10.1016/0165-4608(95)00034-m.

Authors

N Van Roy¹, A Forus, O Myklebost, N C Cheng, R Versteeg, F Speleman

Affiliation

¹ Department of Medical Genetics, University Hospital, Gent, Belgium.

PMID: 7664245
DOI: 10.1016/0165-4608(95)00034-m

Abstract

The neuroblastoma cell line NGP contains two homogeneously staining regions (hsr). One of these hsrs contains MYCN sequences. Reverse painting experiments demonstrated that the second HSR consisted of two chromosome 12-derived amplification units, located at 12q14-15 and 12q24. Southern blot and fluorescence in situ hybridization (FISH) analysis showed amplification of genes located at 12q14-15: SAS, MDM2, and CDK4, GLI, CHOP, CDK2, and A2MR were found not to be amplified. FISH further demonstrated amplification of RSN, a gene located at 12q24. The finding of two distinct chromosome 12 amplification units in a neuroblastoma cell line NGP is reminiscent of recent findings in well-differentiated liposarcoma (WDLPS) and other sarcomas. The second amplification unit on chromosome 12 in NGP is located more distal (12q24) than the one observed in WDLPS (12q21). The mechanism and biologic significance of this amplification process in neuroblastoma and WDLPS remain to be elucidated.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromosomes, Human, Pair 12*
DNA, Neoplasm / analysis
Gene Amplification
Humans
In Situ Hybridization, Fluorescence
Neuroblastoma / genetics*
Tumor Cells, Cultured

Substances

DNA, Neoplasm