Abstract
Objective:
To define precise epitopes on human beta 2-microglobulin (beta 2m) reacting with polyclonal IgM rheumatoid factors (RF) from patients with rheumatoid arthritis (RA).
Methods:
Ten polyclonal RF were tested for their human beta 2m epitope-binding specificities using the entire 99-amino acid sequence synthesized as overlapping 7-mers in an enzyme-linked immunosorbent assay. Glycine substitution for each residue within RF-reacting linear regions was employed to define major reactive sites.
Results and conclusion:
Major beta 2m residues contributing to RF reactivity were tryptophans at positions 60 and 95, lysine at 58, and arginine at position 97.
Publication types
-
Comparative Study
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, Non-P.H.S.
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Amino Acid Sequence
-
Antibody Specificity
-
Arginine / analysis*
-
Arthritis, Rheumatoid / blood
-
Arthritis, Rheumatoid / immunology*
-
Aspartic Acid / analysis
-
Chromatography, Affinity
-
Computers
-
Epitopes / analysis
-
Humans
-
Immunoglobulin A / chemistry
-
Immunoglobulin A / immunology
-
Immunoglobulin Fc Fragments / analysis
-
Immunoglobulin G / chemistry
-
Immunoglobulin G / immunology
-
Immunoglobulin M / chemistry
-
Immunoglobulin M / immunology
-
Isoleucine / analysis
-
Lysine / analysis*
-
Molecular Sequence Data
-
Rheumatoid Factor / analysis*
-
Rheumatoid Factor / immunology
-
Sequence Homology, Amino Acid
-
Tryptophan / analysis*
-
Valine / analysis
-
beta 2-Microglobulin / chemistry*
-
beta 2-Microglobulin / immunology
Substances
-
Epitopes
-
Immunoglobulin A
-
Immunoglobulin Fc Fragments
-
Immunoglobulin G
-
Immunoglobulin M
-
beta 2-Microglobulin
-
Isoleucine
-
Aspartic Acid
-
Tryptophan
-
Rheumatoid Factor
-
Arginine
-
Valine
-
Lysine