Cloning of a full-length cDNA sequence encoding a cdc2-related protein kinase from human endothelial cells

Biochem Biophys Res Commun. 1995 Mar 17;208(2):562-8. doi: 10.1006/bbrc.1995.1375.

Abstract

cDNA clones encoding a novel serine/threonine protein kinase were isolated from human endothelial cell cDNA libraries. The compiled nucleotide sequence is 1757 base pairs in length and contains an open reading frame encoding a 372 amino acid protein, designated C-2k, with a calculated molecular weight of 43 kDa. Sequence analysis indicates that C-2k contains a conserved protein kinase catalytic domain of 308 residues which exhibits its highest sequence identity of 42% to members of the cdc2 kinase family and contains the structural elements characteristic to cdc2-like kinases. C-2k may therefore represent a new member of the cdc2 kinase family.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • Cyclin-Dependent Kinase 9*
  • Cyclin-Dependent Kinases / genetics*
  • DNA Primers / chemistry
  • DNA, Complementary / genetics
  • Endothelium, Vascular / enzymology*
  • Gene Expression
  • Molecular Sequence Data
  • Multigene Family
  • Protein Serine-Threonine Kinases / genetics*
  • Protein Serine-Threonine Kinases / metabolism
  • RNA, Messenger / genetics
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Tissue Distribution

Substances

  • DNA Primers
  • DNA, Complementary
  • RNA, Messenger
  • Protein Serine-Threonine Kinases
  • CDK9 protein, human
  • Cyclin-Dependent Kinase 9
  • Cyclin-Dependent Kinases

Associated data

  • GENBANK/X80230