A new Phlebovirus provisionally named Tunis virus has been isolated from Argas reflexus hermanni ticks parasitizing domestic pigeons. It is the first isolation of an arbovirus from Tunisia and the fourth tick-borne virus to be isolated from the Moghreb following Soldado, Essaouira and Kala Iris in Morocco. The pathogenic potential of this virus is briefly discussed according to the behaviour of its vector and previous serosurveys in the country.