Annexin VI is secreted in human bile

Biochem Biophys Res Commun. 1995 Apr 26;209(3):1039-45. doi: 10.1006/bbrc.1995.1602.

Abstract

A calcium-binding protein of 68 kDa was isolated from human bile that precipitates upon addition of 5 mM CaCl2. This protein was recognized by an immunoaffinity purified anti-annexin VI antibody and it had a similar aminoacid composition as annexin VI. Phospholipase A2 activity was inhibited in vitro in a dose-dependent manner as reported for annexin VI. It is the first time that an annexin secretion is demonstrated in bile.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acids / analysis
  • Animals
  • Annexin A6 / isolation & purification
  • Annexin A6 / metabolism*
  • Annexin A6 / pharmacology
  • Antibodies
  • Bile / chemistry
  • Bile / metabolism*
  • Blotting, Western
  • Calcium-Binding Proteins / isolation & purification
  • Calcium-Binding Proteins / metabolism*
  • Chromatography, Affinity
  • Humans
  • Liver / metabolism
  • Lung / metabolism
  • Pancreas / enzymology
  • Phospholipases A / antagonists & inhibitors
  • Phospholipases A2
  • Rats
  • Swine

Substances

  • Amino Acids
  • Annexin A6
  • Antibodies
  • Calcium-Binding Proteins
  • Phospholipases A
  • Phospholipases A2