Abstract
Fetal rat brown adipocytes show a low number of transforming growth factor beta 1 (TGF-beta 1) binding sites of high affinity, revealing the presence of type I, II and III TGF-beta 1 receptors and a minor-labeled species of approximately 140 kDa. The culture of cells in the presence of TGF-beta 1 induced the expression of the tissue-specific gene uncoupling protein in a dose- and time-dependent manner. In addition, TGF-beta 1 up-regulates the expression of genes involved in adipogenesis such as fatty acid synthase, glycerol 3-phosphate dehydrogenase, malic enzyme and glucose 6-phosphate dehydrogenase, as well as induces the expression of fibronectin (specific target gene for TGF-beta 1). Our results suggest that TGF-beta 1 is a major signal involved in initiating and/or maintaining the thermogenic and adipogenic differentiation of rat fetal brown adipocytes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adipose Tissue, Brown / cytology
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Adipose Tissue, Brown / drug effects*
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Adipose Tissue, Brown / metabolism
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Animals
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Carrier Proteins / genetics
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Cell Differentiation / drug effects
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Cell Differentiation / genetics
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Cells, Cultured
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Fetus
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Fibronectins / genetics
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Gene Expression / drug effects
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Ion Channels
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Kinetics
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Lipid Metabolism
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Membrane Proteins / genetics
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Mitochondrial Proteins
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Rats
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Receptors, Transforming Growth Factor beta / drug effects
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Receptors, Transforming Growth Factor beta / metabolism
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Transforming Growth Factor beta / metabolism
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Transforming Growth Factor beta / pharmacology*
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Uncoupling Agents / metabolism
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Uncoupling Protein 1
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Up-Regulation / drug effects
Substances
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Carrier Proteins
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Fibronectins
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Ion Channels
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Membrane Proteins
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Mitochondrial Proteins
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RNA, Messenger
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Receptors, Transforming Growth Factor beta
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Transforming Growth Factor beta
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Uncoupling Agents
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Uncoupling Protein 1