Functional analysis of the promoter of the Saccharomyces cerevisiae multidrug resistance gene YDR1, which encodes a member of the ATP binding cassette (ABC) superfamily

Biosci Biotechnol Biochem. 1995 Jan;59(1):147-9. doi: 10.1271/bbb.59.147.

Abstract

The Saccharomyces cerevisiae gene YDR1/PDR5/STS1, which encodes a member of the ATP binding cassette (ABC) superfamily, is important for cross-resistance to apparently unrelated drugs. The expression of YDR1 is induced by various drugs and heat shock [Hirata et al., Curr. Genet., 26, 285-294 (1994)]. By deletion analysis of the 5'-noncoding region of the YDR1 gene, two drug responsive regions (-604 to -485 and -335 to -275) were identified with fluphenazine and cycloheximide. Three conserved palindrome sequences were found in these regions.

MeSH terms

  • ATP-Binding Cassette Transporters / genetics*
  • ATP-Binding Cassette Transporters / metabolism
  • Base Sequence
  • Blotting, Northern
  • Cell Membrane / metabolism
  • Drug Resistance, Multiple / genetics*
  • Gene Deletion
  • Gene Expression Regulation, Bacterial / genetics
  • Membrane Proteins / genetics
  • Molecular Sequence Data
  • Molecular Weight
  • Promoter Regions, Genetic*
  • Saccharomyces cerevisiae / genetics*
  • Transcription, Genetic / genetics

Substances

  • ATP-Binding Cassette Transporters
  • Membrane Proteins