Abstract
Three nuclear localization signals (NLS), including an unusual Mat alpha 2-like NLS from maize (Zea mays) R, were found to compete for binding to plant nuclei. In addition, the authentic yeast Mat alpha 2 NLS, which does not function in mammals, was shown to function in plants in vivo. Our results indicate that plants possess a site at the nuclear pore complex that recognizes the three known classes of NLSs.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Cell Nucleus / metabolism
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Cloning, Molecular
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DNA, Plant / genetics
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Genes, Plant
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Molecular Sequence Data
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Nicotiana / genetics
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Nicotiana / metabolism
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Nuclear Localization Signals
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism*
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Plant Proteins / genetics
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Plant Proteins / metabolism*
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Plants / genetics
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Plants / metabolism*
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Plants, Toxic
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Signal Transduction
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Zea mays / genetics
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Zea mays / metabolism
Substances
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DNA, Plant
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Nuclear Localization Signals
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Nuclear Proteins
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Plant Proteins