Enteric adenovirus type 40/41 is considered to be the second major cause of gastroenteritis in young children. In this study fecal specimens 86-123 from diarrhea patients were isolated and examined in Grahm 293 cells. This induced cytopathic effect (CPE) at this cells. Viral particles were also found in fecal specimens and by electron microscopy. Examination of the isolate with Cambridge Biotech Adenoclone-Type 40/41 test kit indicated that it contained EAd. On the basis of the above studies, one strain of EAd was the first isolated virus in China.