Abstract
We have cloned and sequenced the ERCC2/XPD locus of Xiphophorus maculatus. The human ERCC2/XPD gene is a nucleotide excision repair gene presumed to encode an ATP-dependent DNA helicase. The fish ERCC2/XPD gene is represented on 14.5 kb of genomic DNA and is composed of 23 exons. Within the coding regions, the overall nucleotide identity is 74% compared to the human cDNA. Of 760 amino acids compared between human and fish sequences, 127 differences are observed. Of these differences, 48 residues (38%) represent nonconservative amino acid changes, while 79 (62%) are conservative. The majority (73%) of nonconservative differences between the human and the fish amino acid sequences occur in eight distinct groups comprising only about 10% of the total protein. Overall, the fish and human sequences show 83% amino acid identity and 94% similarity when conservative amino acid substitutions are allowed.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenosine Triphosphatases / genetics
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Amino Acid Sequence
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Animals
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Cloning, Molecular
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Conserved Sequence
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Cyprinodontiformes / genetics*
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DNA Helicases / genetics
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DNA Repair
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DNA-Binding Proteins*
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Exons
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Fungal Proteins / genetics
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Humans
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Molecular Sequence Data
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Nuclear Proteins / genetics
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Proteins / genetics*
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RNA Splicing
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Restriction Mapping
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae Proteins
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Schizosaccharomyces / genetics
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Schizosaccharomyces pombe Proteins*
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Sequence Homology, Amino Acid
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Transcription Factors*
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Xeroderma Pigmentosum Group D Protein
Substances
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DNA-Binding Proteins
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Fungal Proteins
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Nuclear Proteins
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Proteins
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Saccharomyces cerevisiae Proteins
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Schizosaccharomyces pombe Proteins
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Transcription Factors
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Rad15 protein, S pombe
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Adenosine Triphosphatases
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Rad3 protein, S cerevisiae
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DNA Helicases
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Xeroderma Pigmentosum Group D Protein
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ERCC2 protein, human