Preparative capillary zone electrophoresis of synthetic peptides conversion of an autosampler into a fraction collector

J Chromatogr A. 1994 Dec 2;686(2):309-17. doi: 10.1016/0021-9673(94)00824-8.

Abstract

Preparative capillary zone electrophoresis of three synthetic peptides was performed either manually or automatically by simple manipulations of a commercial electropherograph that is equipped only with an autosampler without any built-in fraction collection capability. Manual fraction collection was achieved by replacing the outlet (cathode) beaker with a microcentrifuge tube, and automatic fraction collection was accomplished by converting the electropherograph's autosampler into a fraction collector. The latter was easily achieved mainly by the use of an extension wire, which completed the electrical circuit and facilitated fraction collection either at a specified time or within fixed time intervals.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Automation
  • Computers
  • Electrophoresis / instrumentation*
  • Electrophoresis / methods*
  • Peptides / isolation & purification*
  • Spectrophotometry, Ultraviolet

Substances

  • Peptides