Optimization of quantitative culture assay for human immunodeficiency virus from plasma. Plasma Viremia Group Laboratories of the AIDS Clinical Trials Group (National Institute of Allergy and Infectious Diseases)

J Clin Microbiol. 1994 Dec;32(12):3064-7. doi: 10.1128/jcm.32.12.3064-3067.1994.

Abstract

Experimental conditions essential to a simple, reproducible, quantitative human immunodeficiency virus plasma assay were determined. Five parameters were evaluated: length of culture, reproducibility, assay dilution schema, washing procedures, and anticoagulant usage. The recommended quantitative plasma assay utilizes undiluted citrated plasma cultured with peripheral blood mononuclear cells for 14 days with fivefold dilutions and a medium change on day 1 with no washing.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Blood / virology
  • Citric Acid*
  • Glucose / analogs & derivatives
  • HIV Seropositivity / virology
  • HIV-1 / isolation & purification*
  • Humans
  • Microbiological Techniques*
  • Reproducibility of Results

Substances

  • acid citrate dextrose
  • Citric Acid
  • Glucose