The relative radiosensitivity of TK6 and WI-L2-NS lymphoblastoid cells derived from a common source is primarily determined by their p53 mutational status

Mutat Res. 1995 Feb;346(2):85-92. doi: 10.1016/0165-7992(95)90055-1.

Abstract

The lymphoblastoid cell lines WI-L2-NS and TK6 were derived from a non-clonal pool of cells taken from a human spleen. Despite their common background they exhibit marked differences in radiosensitivities; D0 values of 93 and 67 cGy have been reported for WI-L2-NS and TK6 cells respectively. We show here that this differential radiosensitivity is due to a decreased ability of the WI-L2-NS cell line to undergo radiation-induced apoptosis. Further, the WI-L2-NS cell line overexpresses the p53 gene product as a result of a mutation in codon 237 of the p53 gene. These data indicate that WI-L2-NS cells through disruption of normal p53 function are unable to engage the radiation-induced apoptosis program and so are relatively radioresistant.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Apoptosis / genetics*
  • Base Sequence
  • Blotting, Western
  • Cell Line
  • DNA Damage
  • Dose-Response Relationship, Radiation
  • Gamma Rays / adverse effects*
  • Humans
  • Lymphocytes / pathology
  • Lymphocytes / radiation effects*
  • Molecular Sequence Data
  • Mutation
  • Polymerase Chain Reaction
  • Precipitin Tests
  • Radiation Tolerance / genetics*
  • Sequence Analysis, DNA
  • Tumor Suppressor Protein p53 / analysis
  • Tumor Suppressor Protein p53 / genetics*
  • Tumor Suppressor Protein p53 / immunology

Substances

  • Tumor Suppressor Protein p53