No evidence of mycoplasmas in peripheral blood mononuclear cell fraction of HIV-infected patients

F van Kuppeveld; W Melchers; J Kissing; J van der Logt; J Galama

doi:10.1007/BF01716704

No evidence of mycoplasmas in peripheral blood mononuclear cell fraction of HIV-infected patients

Infection. 1994 May-Jun;22(3):204-6. doi: 10.1007/BF01716704.

Authors

F van Kuppeveld¹, W Melchers, J Kissing, J van der Logt, J Galama

Affiliation

¹ Dept. of Microbiology, University of Nijmegen, The Netherlands.

PMID: 7927818
DOI: 10.1007/BF01716704

Abstract

In this study, the prevalence of mycoplasmas in peripheral blood mononuclear cells (PBMC) from HIV-infected individuals was investigated using a mycoplasma genus-specific PCR assay. No mycoplasmas were detected in the PBMC samples from any of the 25 HIV-infected individuals (CDC 2, n = 8; CDC 3, n = 2; CDC 4, n = 15) or ten HIV-seronegative controls. As an internal control, HIV specific sequences were detected in the samples from all HIV-seropositives. These negative results do not support a suggested role of mycoplasmas as co-factor in the progression of HIV infection towards AIDS.

MeSH terms

AIDS-Related Opportunistic Infections / blood
AIDS-Related Opportunistic Infections / complications
AIDS-Related Opportunistic Infections / epidemiology*
Base Sequence
Case-Control Studies
DNA, Bacterial*
Female
Humans
Leukocytes, Mononuclear / microbiology*
Male
Molecular Sequence Data
Mycoplasma / genetics*
Mycoplasma Infections / blood*
Mycoplasma Infections / complications
Mycoplasma Infections / epidemiology
Polymerase Chain Reaction
Prevalence
Risk Factors

Substances

DNA, Bacterial