Abstract
Retinal S-antigen and interphotoreceptor retinoid-binding protein (IRBP) were purified by DEAE-Sephacel ionexchange chromatography and Con A Sepharose affinity chromatography. Amino acid analysis of the S-antigen and IRBP was carried out and compared with that presented by others. The possible reason for difference with regard to some amino acids and related problems were analyzed and discussed.
MeSH terms
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Amino Acids / analysis*
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Animals
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Antigens / chemistry*
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Antigens / isolation & purification
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Arrestin
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Autoantigens / chemistry
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Autoantigens / isolation & purification
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Cattle
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Chromatography, Affinity
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Chromatography, Ion Exchange
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Eye Proteins / chemistry*
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Eye Proteins / isolation & purification
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Phosphodiesterase Inhibitors / chemistry
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Phosphodiesterase Inhibitors / isolation & purification
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Retina / chemistry*
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Retinol-Binding Proteins / chemistry*
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Retinol-Binding Proteins / isolation & purification
Substances
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Amino Acids
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Antigens
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Arrestin
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Autoantigens
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Eye Proteins
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Phosphodiesterase Inhibitors
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Retinol-Binding Proteins
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interstitial retinol-binding protein