The tracheal mucosa of fourteen dogs was denuded and resected in sizes of 20mm x 25mm respectively. The raw surfaces were managed with mucosal flaps. The healing process of denuded area was observed by microscope, light microscope and scanning electron microscope. The outcomes were compared with those of control group. From this experiment it was demonstrated that the surgical approach could effectively promote epithelial healing of denuded surfaces, prevent formation of cicatricial stenosis in trachea and get an excellent epithelialization on the wounds.