Persistence of a rheumatoid factor (RF)-producing B cell clone with a somatically mutated Ig kappa chain in a patient with rheumatoid arthritis

Clin Exp Immunol. 1994 Aug;97(2):200-3. doi: 10.1111/j.1365-2249.1994.tb06068.x.

Abstract

The V kappa IV gene encoding the light chain of an IgA has been shown to have undergone 31 somatic mutations compared with the single existing V kappa IV germ-line gene. We now show the persistence of the rearranged and mutated DNA coding for this RF over a period of 5 years in the peripheral blood lymphocytes (PBL) of the patient with rheumatoid arthritis (RA). The sequence of the RF has been conserved to identity over this period. These results raise the possibility that the particular antigenic stimulus leading to RF production in this RA patient is active over a long period of time.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arthritis, Rheumatoid / genetics
  • Arthritis, Rheumatoid / immunology*
  • B-Lymphocytes / immunology*
  • Base Sequence
  • Clone Cells
  • Cloning, Molecular
  • Gene Rearrangement, B-Lymphocyte, Light Chain / genetics*
  • Humans
  • Immunoglobulin kappa-Chains / genetics*
  • Molecular Sequence Data
  • Mutation / genetics*
  • Polymerase Chain Reaction
  • Rheumatoid Factor / genetics*

Substances

  • Immunoglobulin kappa-Chains
  • Rheumatoid Factor