Sphinganine potentiation of dimethyl sulfoxide-induced granulocyte differentiation, increase of alkaline phosphatase activity and decrease of protein kinase C activity in a human leukemia cell line (HL-60)

Biochem Biophys Res Commun. 1994 Mar 15;199(2):888-96. doi: 10.1006/bbrc.1994.1312.

Abstract

The differentiation of HL-60 promyelocytic cells toward mature granulocytic cells induced by dimethyl sulfoxide (DMSO) was accompanied by a quantitative similar increase in alkaline phosphatase (ALP) activity and decrease in protein kinase C (PKC) activity. The combination of DMSO and sphinganine (SP), a potent inhibitor of PKC, increased in parallel the percentage of mature cells and the ALP activity. The enhancement of DMSO-induced differentiation and the potentiation of the decrease of PKC activity by SP also seemed to correlate with each other. Our results indicate that both ALP and PKC may play a role in the DMSO-induced granulocytic differentiation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaline Phosphatase / isolation & purification
  • Alkaline Phosphatase / metabolism*
  • Cell Differentiation / drug effects*
  • Cell Line
  • Dimethyl Sulfoxide / pharmacology*
  • Drug Synergism
  • Granulocytes / cytology*
  • Granulocytes / drug effects
  • Humans
  • Kinetics
  • Leukemia, Promyelocytic, Acute
  • Protein Kinase C / isolation & purification
  • Protein Kinase C / metabolism*
  • Sphingosine / analogs & derivatives*
  • Sphingosine / pharmacology
  • Time Factors
  • Tumor Cells, Cultured

Substances

  • Protein Kinase C
  • Alkaline Phosphatase
  • Sphingosine
  • safingol
  • Dimethyl Sulfoxide