Analysis of 6 VNTR loci by 'multiplex' PCR and automated fluorescent detection

Hum Genet. 1993 Dec;92(6):554-62. doi: 10.1007/BF00420938.

Abstract

The polymerase chain reaction was used to amplify six small variable number of tandem repeat loci in two reactions (D19S20 co-amplifying with D17S5 and D1S80; D17S766 co-amplifying with D16S83 and D17S24). When coupled with fluorescent detection of the products, this provides a rapid, highly discriminating automated test. Preferential amplification of small alleles, leading to 'allelic dropout' was found to occur in D19S20 and D16S83. Population databases are presented for Caucasians and Afro-Caribbeans at loci D19S20, D16S83 and D17S24, and for Asians at D19S20.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Apolipoproteins B / genetics*
  • Base Sequence
  • DNA / genetics*
  • Electrophoresis, Polyacrylamide Gel
  • Genetic Markers
  • Genetics, Population
  • Humans
  • Molecular Sequence Data
  • Phenotype
  • Polymerase Chain Reaction / methods*
  • Repetitive Sequences, Nucleic Acid / genetics*

Substances

  • Apolipoproteins B
  • Genetic Markers
  • DNA