Effect of spacer groups connecting insulin with fluorophore in Gly(A1)-sulfobenzoxadiazole-labeled insulins on the immunoreactivity toward anti-insulin antibody

Biol Pharm Bull. 1993 May;16(5):516-7. doi: 10.1248/bpb.16.516.

Abstract

The immunoreactivity (specific binding) of three Gly(A1)-sulfobenzoxadiazole-labeled insulins which have different spacer groups, Gly(A1)-[3-(4-sulfobenzoxadiazol-7-ylthio)propanoyl]-insulin , Gly(A1)-[2-(4-sulfobenzoxadiazol-7-ylthio)acetyl]-insulin, Gly(A1)-[3-carboxy-2(or 3)-(4-sulfobenzoxadiazol-7-ylthio)propanoyl]-insulin, toward an antiporcine insulin monoclonal antibody was investigated, where solid-phase fluoroimmunoassay technique was utilized. The immunoreactivities of three labeled insulins were 2.1, 1.8 and 1 in that order.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Chromatography, High Pressure Liquid
  • Fluorescent Antibody Technique
  • Fluorescent Dyes
  • Indicators and Reagents
  • Insulin / analogs & derivatives*
  • Insulin / chemical synthesis
  • Insulin / chemistry*
  • Insulin / immunology
  • Insulin Antibodies / immunology*
  • Swine

Substances

  • Antibodies, Monoclonal
  • Fluorescent Dyes
  • Indicators and Reagents
  • Insulin
  • Insulin Antibodies