Abstract
Two monoclonal antibodies (SPM-1 and SPM-2) immunoprecipitate brain N-type calcium channels. On immunoaffinity chromatography of digitonin extracts of bovine brain membranes on SPM-1- and SPM-2-Sepharose, proteins of 36 (syntaxins A and B), 28 and 19 kDa are specifically retained by both columns. Here we show that the 19 and 28 kDa bands contain VAMP/synaptobrevin-2, and rab3A/smg25A and SNAP-25, respectively. Since SPM-1 and SPM-2 recognize only syntaxins and the 28 kDa band (rab3A/sm25A and SNAP-25), respectively, the results indicate that all these proteins form a complex. Our results suggest tight linkage between the components involved in neurotransmitter release.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antigens, Surface / analysis*
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Antigens, Surface / chemistry
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Brain Chemistry*
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Cattle
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Electrophoresis, Polyacrylamide Gel
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GTP-Binding Proteins / analysis*
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GTP-Binding Proteins / chemistry
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Membrane Proteins / analysis*
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Membrane Proteins / chemistry
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Molecular Sequence Data
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Nerve Tissue Proteins / analysis*
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Nerve Tissue Proteins / chemistry
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R-SNARE Proteins
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Synapses / chemistry*
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Synaptosomal-Associated Protein 25
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Syntaxin 1
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rab3 GTP-Binding Proteins
Substances
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Antigens, Surface
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Membrane Proteins
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Nerve Tissue Proteins
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R-SNARE Proteins
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Synaptosomal-Associated Protein 25
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Syntaxin 1
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GTP-Binding Proteins
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rab3 GTP-Binding Proteins