A complex of rab3A, SNAP-25, VAMP/synaptobrevin-2 and syntaxins in brain presynaptic terminals

FEBS Lett. 1993 Sep 13;330(2):236-40. doi: 10.1016/0014-5793(93)80281-x.

Abstract

Two monoclonal antibodies (SPM-1 and SPM-2) immunoprecipitate brain N-type calcium channels. On immunoaffinity chromatography of digitonin extracts of bovine brain membranes on SPM-1- and SPM-2-Sepharose, proteins of 36 (syntaxins A and B), 28 and 19 kDa are specifically retained by both columns. Here we show that the 19 and 28 kDa bands contain VAMP/synaptobrevin-2, and rab3A/smg25A and SNAP-25, respectively. Since SPM-1 and SPM-2 recognize only syntaxins and the 28 kDa band (rab3A/sm25A and SNAP-25), respectively, the results indicate that all these proteins form a complex. Our results suggest tight linkage between the components involved in neurotransmitter release.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens, Surface / analysis*
  • Antigens, Surface / chemistry
  • Brain Chemistry*
  • Cattle
  • Electrophoresis, Polyacrylamide Gel
  • GTP-Binding Proteins / analysis*
  • GTP-Binding Proteins / chemistry
  • Membrane Proteins / analysis*
  • Membrane Proteins / chemistry
  • Molecular Sequence Data
  • Nerve Tissue Proteins / analysis*
  • Nerve Tissue Proteins / chemistry
  • R-SNARE Proteins
  • Synapses / chemistry*
  • Synaptosomal-Associated Protein 25
  • Syntaxin 1
  • rab3 GTP-Binding Proteins

Substances

  • Antigens, Surface
  • Membrane Proteins
  • Nerve Tissue Proteins
  • R-SNARE Proteins
  • Synaptosomal-Associated Protein 25
  • Syntaxin 1
  • GTP-Binding Proteins
  • rab3 GTP-Binding Proteins