Bowringia mildbraedii agglutinin: polypeptide composition, primary structure and homologies with other legume lectins

Biochim Biophys Acta. 1993 Sep 3;1202(1):38-46. doi: 10.1016/0167-4838(93)90060-5.

Abstract

The amino-acid sequences of the subunits of the lectin BMA from seeds of Bowringia mildbraedii have been determined. The data indicate that the lectin consists of a precursor polypeptide of approx. 29 kDa that is cleaved almost completely into two fragments of approx. 13.3 kDa (alpha subunit) and approx. 11.9 kDa (beta subunit), respectively. The beta subunit represents the N-terminal half of precursor polypeptides and is disulphide-linked in a beta beta dimer in the native (alpha beta)2 protein. BMA shows extensive amino-acid sequence homologies with known legume lectins. The site of post-translational proteolysis of the putative precursor occurs at a position similar to that identified in lectins obtained from other Sophoreae plants such as Sophora japonica and in Diocleae lectins such as Concanavalin A, but different from that of two chain lectins obtained from other tribes of the Papilionaceae.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agglutinins / chemistry*
  • Amino Acid Sequence
  • Amino Acids / analysis
  • Binding Sites
  • Concanavalin A / chemistry
  • Disulfides
  • Fabaceae / chemistry*
  • Molecular Sequence Data
  • Plant Lectins
  • Plants, Medicinal*
  • Protein Precursors / chemistry*
  • Sequence Homology, Amino Acid

Substances

  • Agglutinins
  • Amino Acids
  • Disulfides
  • Plant Lectins
  • Protein Precursors
  • Concanavalin A