Crystallization and preliminary crystallographic study of cytochrome cd1 nitrite reductase from Thiosphaera pantotropha

J Mol Biol. 1993 Aug 20;232(4):1211-2. doi: 10.1006/jmbi.1993.1473.

Abstract

Single crystals of cytochrome cd1 nitrite reductase from Thiosphaera pantotropha have been grown in the presence of ammonium sulphate by vapour diffusion techniques. Crystals are suitable for X-ray analysis and diffract to dmin = 1.5 A. They belong to space group P2(1) with unit cell dimensions of a = 107.8 A, b = 61.9 A, c = 101.2 A, beta = 112.5 degrees. The crystallographic asymmetric unit contains a dimer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteria, Aerobic / enzymology*
  • Crystallization
  • Cytochromes
  • Electron Transport Complex IV / chemistry*
  • Gram-Negative Chemolithotrophic Bacteria / enzymology*
  • Nitrite Reductases*
  • X-Ray Diffraction

Substances

  • Cytochromes
  • Nitrite Reductases
  • nitrite reductase (NO-forming)
  • Electron Transport Complex IV