Abstract
We have developed a method that allows reliable construction of high quality FISH probes from yeast artificial chromosomes (YACs) based on the separation of YACs by pulse-field gel electrophoresis and a rapid sequence-independent amplification procedure (SIA). These probes can be used to localize YACs on metaphase chromosomes and also with high efficiency, in interphase nuclei.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Sequence
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Chromosome Mapping
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Chromosomes, Artificial, Yeast*
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DNA Primers
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DNA Probes
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Electrophoresis, Gel, Pulsed-Field
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Humans
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In Situ Hybridization, Fluorescence / methods*
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Molecular Sequence Data
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Polymerase Chain Reaction / methods*