Thiol and disulfide determination by free zone capillary electrophoresis

J Biochem Biophys Methods. 1993 Jul;26(4):335-41. doi: 10.1016/0165-022x(93)90034-l.

Abstract

A rapid, sensitive and simple method for the determination of reduced and oxidized glutathione, cysteine, cystine, cysteamine, cystamine and their respective mixed disulfides is described. The compounds were separated and identified in a single step by capillary zone electrophoresis. The method was used to follow the thiol-disulfide interconversion and to measure glutathione levels in lens extracts.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cattle
  • Cystamine / analysis
  • Cysteamine / analysis
  • Cysteine / analysis
  • Cystine / analysis
  • Disulfides / analysis*
  • Dithiothreitol / analysis
  • Electrophoresis / methods*
  • Glutathione / analogs & derivatives
  • Glutathione / analysis
  • Glutathione Disulfide
  • Oxidation-Reduction
  • Sensitivity and Specificity
  • Sulfhydryl Compounds / analysis*

Substances

  • Disulfides
  • Sulfhydryl Compounds
  • Cystine
  • Cysteamine
  • Glutathione
  • Cysteine
  • Cystamine
  • Dithiothreitol
  • Glutathione Disulfide